How to create an HTS report
1. Identify hitsPaste primary screening activity data and select a threshold.
2. Check assay qualityCalculate Z-prime, SSMD, signal window and CV from controls.
3. Fit IC50Run 4PL dose-response fitting and inspect curve quality.
4. PrioritizeCombine potency, selectivity, tractability and artifact flags.
Use this as research screening analytics. Confirm hits with replicates, orthogonal assays, counter-screens,
dose-response confirmation, PAINS/tox filters, and medicinal chemistry review.
Scientific guardrails
Primary HTS hit calls are not final leads. Assay quality, artifacts, counter-screening, reproducibility,
curve quality, and chemical tractability must drive decisions.
Z-prime
SSMD
4PL IC50
Counter-screen
Replicate QC
User outputs
Hit table
Dose-response curve
Assay QC
Plate map
JSON / HTML / Word / CSV
Share report
Primary Screening Data
Hit Distribution
Run hit identification to see hit rate, classes, and distribution.
Assay Quality Controls
QC Interpretation
Z-prime ≥ 0.5 is generally suitable for HTS. Combine Z-prime with SSMD, CV, signal window, and control trends.
Dose-Response 4PL
Dose-Response Curve
Batch Dose-Response JSON
Plate Normalization
Plate Map View
Counter-screen Selectivity
Counter-screen Logic
Prioritize compounds with strong primary activity and low counter-screen activity. Counter-screen hits may indicate assay artifacts,
nonspecific activity, cytotoxicity, aggregation, or promiscuity.
Multi-Parameter Hit Prioritization
Complete Report Center
Create a complete HTS analytics report, then view it here or download/share it.
Raw JSON
{}